Regulatory T Cells Modulate CD4 Proliferation after Severe Trauma via IL-10

Sturm, Ramona; Xanthopoulos, Lara; Heftrig, David; Oppermann, Elsie; Vrdoljak, Teodora; Dunay, Ildiko Rita; Marzi, Ingo; Relja, Borna

doi:10.3390/jcm9041052

by Ramona Sturm, Lara Xanthopoulos, David Heftrig, Elsie Oppermann, Teodora Vrdoljak, Ildiko Rita Dunay, Ingo Marzi, Borna Relja

Abstract:

OBJECTIVE: Severely injured patients frequently develop an immunological imbalance following the traumatic insult, which might result in infectious complications evoked by a persisting immunosuppression. Regulatory T cells (Tregs) maintain the immune homeostasis by suppressing proinflammatory responses, however, their functionality after trauma is unclear. Here, we characterized the role of Tregs in regulating the proliferation of CD4(+) lymphocytes in traumatized patients (TP). METHODS: Peripheral blood was obtained daily from 29 severely injured TP (Injury Severity Score, ISS ≥16) for ten days following admission to the emergency department (ED). Ten healthy volunteers (HV) served as controls. The frequency and activity of Tregs were assessed by flow cytometry. Proliferation of CD4(+) cells was analyzed either in presence or absence of Tregs, or after blocking of either IL-10 or IL-10R1. RESULTS: The frequencies of CD4(+)CD25(high) and CD4(+)CD25(+)CD127(-) Tregs were significantly decreased immediately upon admission of TP to the ED and during the following 10 post-injury days. Compared with HV CD4(+) T cell proliferation in TP increased significantly upon their admission and on the following days. As expected, CD4(+)CD25(+)CD127(-) Tregs reduced the proliferation of CD4(+) cells in HV, nevertheless, CD4(+) proliferation in TP was increased by Tregs. Neutralization of IL-10 as well as blocking the IL-10R1 increased further CD4(+) T cell proliferation in Tregs-depleted cultures, thereby confirming an

Reference:

Regulatory T Cells Modulate CD4 Proliferation after Severe Trauma via IL-10 (Ramona Sturm, Lara Xanthopoulos, David Heftrig, Elsie Oppermann, Teodora Vrdoljak, Ildiko Rita Dunay, Ingo Marzi, Borna Relja), In Journal of clinical medicine, volume 9, 2020.

Bibtex Entry:

@article{sturm_regulatory_2020,
	title = {Regulatory {T} {Cells} {Modulate} {CD}4 {Proliferation} after {Severe} {Trauma} via {IL}-10},
	volume = {9},
	issn = {2077-0383 2077-0383 2077-0383},
	doi = {10.3390/jcm9041052},
	abstract = {OBJECTIVE: Severely injured patients frequently develop an immunological imbalance  following the traumatic insult, which might result in infectious complications  evoked by a persisting immunosuppression. Regulatory T cells (Tregs) maintain the  immune homeostasis by suppressing proinflammatory responses, however, their  functionality after trauma is unclear. Here, we characterized the role of Tregs in  regulating the proliferation of CD4(+) lymphocytes in traumatized patients (TP).  METHODS: Peripheral blood was obtained daily from 29 severely injured TP (Injury  Severity Score, ISS ≥16) for ten days following admission to the emergency  department (ED). Ten healthy volunteers (HV) served as controls. The frequency and  activity of Tregs were assessed by flow cytometry. Proliferation of CD4(+) cells was  analyzed either in presence or absence of Tregs, or after blocking of either IL-10  or IL-10R1. RESULTS: The frequencies of CD4(+)CD25(high) and CD4(+)CD25(+)CD127(-)  Tregs were significantly decreased immediately upon admission of TP to the ED and  during the following 10 post-injury days. Compared with HV CD4(+) T cell  proliferation in TP increased significantly upon their admission and on the  following days. As expected, CD4(+)CD25(+)CD127(-) Tregs reduced the proliferation  of CD4(+) cells in HV, nevertheless, CD4(+) proliferation in TP was increased by  Tregs. Neutralization of IL-10 as well as blocking the IL-10R1 increased further  CD4(+) T cell proliferation in Tregs-depleted cultures, thereby confirming an},
	language = {eng},
	number = {4},
	journal = {Journal of clinical medicine},
	author = {Sturm, Ramona and Xanthopoulos, Lara and Heftrig, David and Oppermann, Elsie and Vrdoljak, Teodora and Dunay, Ildiko Rita and Marzi, Ingo and Relja, Borna},
	month = apr,
	year = {2020},
	pmid = {32276346},
	pmcid = {PMC7230720},
	keywords = {IL-10, Lymphocytes, polytrauma, proliferation, regulatory T cells}
}