M1 But Not M0 Extracellular Vesicles Induce Polarization of RAW264.7 Macrophages Via the TLR4-NFκB Pathway In Vitro

Shi, Yulong; Luo, Peng; Wang, Weikang; Horst, Klemens; Bläsius, Felix; Relja, Borna; Xu, Ding; Hildebrand, Frank; Greven, Johannes

doi:10.1007/s10753-020-01236-7

by Yulong Shi, Peng Luo, Weikang Wang, Klemens Horst, Felix Bläsius, Borna Relja, Ding Xu, Frank Hildebrand, Johannes Greven

Abstract:

In response to different stimuli (e.g., infections), naive macrophages polarize into M1 macrophages, which have the potential to secrete numerous pro-inflammatory cytokines and extracellular vesicles (EVs). EVs are important mediators of intercellular communication. Via horizontal transfer, EVs transport various molecules (e.g., proteins, DNA, and RNA) to target cells. This in vitro study elucidated that M1-EVs from macrophages induced by interferon-γ (IFN-γ) and lipopolysaccharide (LPS) 24 h (M1), but not M0-EVs from untreated macrophages (M0), shifted M0 into M1 phenotype via activating the nuclear factor-κB pathway. The characteristics of these EVs were assessed by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and a western blot assay. RAW 264.7 cells were incubated with M1-EVs (experimental group) or PBS (sham group) or M0-EVs (control group) for 24 h. The viability, change of shape, and phenotype differentiation of the macrophages were identified by a

Reference:

M1 But Not M0 Extracellular Vesicles Induce Polarization of RAW264.7 Macrophages Via the TLR4-NFκB Pathway In Vitro (Yulong Shi, Peng Luo, Weikang Wang, Klemens Horst, Felix Bläsius, Borna Relja, Ding Xu, Frank Hildebrand, Johannes Greven), In Inflammation, volume 43, 2020.

Bibtex Entry:

@article{shi_m1_2020,
	title = {M1 {But} {Not} {M}0 {Extracellular} {Vesicles} {Induce} {Polarization} of {RAW}264.7 {Macrophages} {Via}  the {TLR}4-{NFκB} {Pathway} {In} {Vitro}},
	volume = {43},
	issn = {1573-2576 0360-3997 0360-3997},
	doi = {10.1007/s10753-020-01236-7},
	abstract = {In response to different stimuli (e.g., infections), naive macrophages polarize into  M1 macrophages, which have the potential to secrete numerous pro-inflammatory  cytokines and extracellular vesicles (EVs). EVs are important mediators of  intercellular communication. Via horizontal transfer, EVs transport various  molecules (e.g., proteins, DNA, and RNA) to target cells. This in vitro study  elucidated that M1-EVs from macrophages induced by interferon-γ (IFN-γ) and  lipopolysaccharide (LPS) 24 h (M1), but not M0-EVs from untreated macrophages (M0),  shifted M0 into M1 phenotype via activating the nuclear factor-κB pathway. The  characteristics of these EVs were assessed by transmission electron microscopy  (TEM), nanoparticle tracking analysis (NTA), and a western blot assay. RAW 264.7  cells were incubated with M1-EVs (experimental group) or PBS (sham group) or M0-EVs  (control group) for 24 h. The viability, change of shape, and phenotype  differentiation of the macrophages were identified by a},
	language = {eng},
	number = {5},
	journal = {Inflammation},
	author = {Shi, Yulong and Luo, Peng and Wang, Weikang and Horst, Klemens and Bläsius, Felix and Relja, Borna and Xu, Ding and Hildebrand, Frank and Greven, Johannes},
	month = oct,
	year = {2020},
	pmid = {32323096},
	pmcid = {PMC7476919},
	keywords = {extracellular vesicles, M1 macrophages, NF-κB pathway, polarization, RAW264.7 macrophages},
	pages = {1611--1619}
}